Cloning and sequencing of the ovine growth hormone gene
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چکیده
منابع مشابه
RT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli
Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...
متن کاملCloning, sequencing, and polymorphisms of the Wistar-Imamichi rat growth hormone gene using PCR-SSCP.
We successfully cloned the Wistar-Imamichi (WI) rat growth hormone gene (GenBank accession: GQ890681), which contained 5 exons and 4 introns. Using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique, a novel missense substitution single nucleotide polymorphism was identified and tested for Hardy-Weinberg equilibrium with the χ(2) test. This gene fragment ...
متن کاملIDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS
Background and Objectives: Annexin is the common name for genes and proteins that were identified as calcium-dependent phospholipid-binding proteins. Recently a more complex set of functions has been recognized for this superfamily of proteins including in vesicle trafficking, cell division, apoptosis, calcium signalling, mineralization, crystal nucleation inside the extracellular organelle...
متن کاملP-191: Cloning and Expression of Recombinant Ovine FSH Hormone in Pichia Pastoris
Background: Follicle stimulating hormone is a heterodimeric protein composed of two subunits, α and ß, which are linked noncovalently. The hypophysial gonadotropin FSH plays an important role in the regulation of oocyte maturation, and a key component for growth of ovulator follicles in ewes. Materials and Methods: This study seeks to clone and express the ovine follicle stimulating hormone sub...
متن کاملacipenser persicus growth hormone gene sequencing and its structures
administering growth hormone increases growth rate of cultured fish. the aim of this study was isolation and synthesize of persian sturgeon gh cdna. the total rna was extracted from pituitary gland of persian sturgeon and cdna was synthesized. the full-length cdna sequence of persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. the position of...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.18.9046